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"SWEET RELIEF . . . Injecting tendons with a dextrose
(sugar) solution to trigger
an inflammatory reaction is
just one way in which
which practitioners are using prolotherapy to target
sprains and strainsprains and strains."

K. Dean Reeves, MD
BioMechanics Magazine
Sept. 2004

Better Business Bureau MemberSM

Prolozone Therapy Research

Oxidants Stimulate Growth Factors

Rao GN. Division of Cardiology, University of Texas Medical Branch, Galveston 77555, USA. Oncogene. 1996 Aug 15; 13(4):713-9.

  • “Protein tyrosine phosphorylation events play determinant roles in cellular processes such as proliferation and differentiation.”

  • "We have recently reported that hydrogen peroxide, an active oxygen species and a cellular oxidant, stimulates growth response events in vascular smooth muscle cells. Our findings show that hydrogen peroxide stimulates tyrosine phosphorylation of several proteins including epidermal growth factor receptor.”

Oxidants Activate Growth Factor Receptors

Kai Chen, Shane R. Thomas, et al. Mitochondrial function is required for hydrogen peroxide-induced growth factor receptor transactivation and downstream signaling. J. Biol. Chem. 279(33):35079-35086.

  • “These data establish a novel role for the mitochondrion as a proximal target specific to hydrogen peroxide-induced signaling and growth factor transactivation.”

Oxidants Mediate the Effects of Growth Factors

Ioan Cucoranu, Roza Clempus. Circulation Research. 2005;97:900-907.

  • “NAD(P)H oxidadase 4 mediates transforming growth factor-beta1-induced differentiation of cardiac fibroblasts into myofibroblasts.”

“Intraforaminal O(2)-O(3) Versus Periradicular Steroidal Infiltrations in Lower Back Pain: Randomized Controlled Study.”

Bonetti M, Fontana A, Cotticelli B, Volta GD, Guindani M, Leonardi M.. Am J Neuroradiol. 2005 May; 26(5):996-1000.

  • “CONCLUSION: Oxygen-ozone treatment was highly effective in relieving acute and chronic lower back pain and sciatica. The gas mixture can be administered as a first treatment to replace epidural steroids.”

“Painkilling Effect of Ozone-Oxygen Injection on Spine and Joint Osteoarthritis.”

Al-Jaziri AA, Mahmoodi SM, Saudi Med J. 2008 April;29(4):553-7. Departments of Surgery, Rashid Hospital, Dubai, United Arab Emirates.

  • “CONCLUSION: This study validates the pain killing effect of ozone-oxygen injection on osteoarthritis of the joints and spine. Its long-term effect on pain advocates the likelihood of some histological changes as mechanism of its action.”

“Intramuscular Oxygen-Ozone Therapy in the Treatment of Acute Back Pain with Lumbar Disc Herniation-a Multi-Center, Randomized, Double-Blind, Clinical Trial of Active and Simulated Lumbar Paravertebral Injection.”

Marco Paoloni, MD, Luca Di Sante, MD, Angelo Cacchio, MD, et al., SPINE Volume 34, Number 13, pp 1337-1344, 2009.

  • “It should, nevertheless, be pointed out that the pathophysiology of LBP and radicular pain has not yet been fully understood. Indeed, the mechanical effect of nerve root compression does not seem to completely explain radicular pain. Inflammatory changes have been observed in lumbar disc and periradicular tissues in people suffering from lumbar and radiating pain, and a correlation has been found between inflammatory changes in herniated lumbar disc specimen and outcome after lumbar disc surgery, with a lower VAS rating in those patients showing inflammatory changes in the herniated disc after surgery.”

  • Ozone vs placebo – 61% vs. 33% pain free

Fifth International Symposium on the Applications of Ozone, April 2007, Havana, Cuba

  •  “We conclude that intra-articular ozone is an effective therapy in the treatment of grade III knee osteoarthritis resistant to treatment with NSAIDS.”

“Ozone Shot as Effective as Surgery for Herniated Disc."

As reported in CyberRounds, by Neil Wagner online at

A Toronto team examined the results of 12 previous studies involving over 8,000 patients of ozone treatment of herniated discs. The studies showed ozone therapy to be just as effective as surgery but with a shorter recovery time and a much lower risk of complications. 

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